Simultaneous detection of antibodies to five simian viruses in nonhuman primates using recombinant viral protein based multiplex microbead immunoassays

► Detection of Simian virus infection is important in nonhuman primates, especially in specific pathogen free colonies. ► Assays using recombinant viral protein antigens are as accurate as viral lysate based assays. ► Multiplex recombinant viral protein antigen based assays are potentially more efficient, less biohazardous, less variable, and less expensive than traditional assays.