Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134932 | Journal of Virological Methods | 2012 | 6 Pages |
Abstract
⺠A real-time PCR assay based on the TaqMan chemistry was developed for detection and quantitation of squash leaf curl virus (SLCV) in melon and squash plants. ⺠This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. ⺠The protocol of real-time PCR established in this study enabled detection of as little as 102 copies of SLCV DNA with the CP gene as the target.
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Authors
Cheng-Ping Kuan, Hung-Chang Huang, Chia-Che Chang, Yi-Lin Lu,