Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134974 | Journal of Virological Methods | 2011 | 7 Pages |
Abstract
⺠Universal real-time PCR primer pair demonstrated to hybridize to and detect each of the known Lyssaviruses (including Rabies virus) with greater sensitivity than a standard pan-Lyssavirus hemi-nested RT-PCR typically used. ⺠Target sequences of bat derived virus species unavailable for analysis (Aravan-, Khujand-, Irkut-, West Caucasian bat- and Shimoni bat virus) were synthesized to produce oligonucleotides and the synthetic DNA was used as a target for primer hybridization.
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Authors
David T.S. Hayman, Ashley C. Banyard, Philip R. Wakeley, Graeme Harkess, Denise Marston, James L.N. Wood, Andrew A. Cunningham, Anthony R. Fooks,