Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6135064 | Journal of Virological Methods | 2011 | 8 Pages |
Abstract
⺠We compared one-step and two-step RT-pPCR methods to quantify HIV-2 mRNA transcripts. ⺠These are important to investigate low HIV-2 viremia despite high proviral DNA. ⺠The two-step RT-qPCR provides improved detection of low level HIV-2 gag and tat mRNA.
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Authors
Rui S. Soares, Paula Matoso, Marta Calado, Ana E. Sousa,