Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6135163 | Journal of Virological Methods | 2012 | 6 Pages |
Abstract
⺠We developed the rapid detection method for RNA viruses using real time PCR. ⺠We synthesized the cDNA from RNA viruses at different time points in order to show the reduced detection time. ⺠cDNA synthesis for 5 min was sufficient for detection of RNA virus. ⺠Detection of RNA viruses was completed within 22 min including reverse transcription and real time PCR.
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Authors
Mi-Sun Yoo, Kim Cuc Nguyen Thi, Phu Van Nguyen, Sang-Hoon Han, Soon-Hwan Kwon, Byoung-Su Yoon,