Use of PCR-based assays for the detection of the adventitious agent porcine circovirus type 1 (PCV1) in vaccines, and for confirming the identity of cell substrates and viruses used in vaccine production

► The use of PCRs and RT-PCRs in assays to assess the quality of viral vaccines was evaluated. ► PCRs were standardized for the detection of PCV1, and to ascertain the identity of cells. ► RT-PCRs were standardized to ascertain the identity of viruses. ► Several vaccines were tested, and passed identity and contamination testing. ► The tests can be used for in-process and quality control assays.