Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6135173 | Journal of Virological Methods | 2012 | 7 Pages |
Abstract
⺠A novel serum neutralization test for Nipah virus (NiV) infection was developed. ⺠This assay employs SEAP-expressing VSV pseudotype bearing NiV-F/G proteins. ⺠It is performed by measuring SEAP in supernatant using an ELISA plate reader. ⺠Various species sera were tested and showed identical serostatus to live NiV test. ⺠The assay is useful for high-throughput screening for NiV neutralizing antibodies.
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Authors
Yoshihiro Kaku, Akira Noguchi, Glenn A. Marsh, Jennifer A. Barr, Akiko Okutani, Kozue Hotta, Boldbaatar Bazartseren, Shuetsu Fukushi, Christopher C. Broder, Akio Yamada, Satoshi Inoue, Lin-Fa Wang,