Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6135782 | Microbes and Infection | 2012 | 5 Pages |
Abstract
In this study we investigated the role of nitric oxide (NO) in monocyte fungicidal activity against Paracoccidioides brasiliensis. We found that cells primed with IFN-γ, TNF-α or GM-CSF and challenged with a high-(Pb18) or low-virulence (Pb265) strain of the fungus increase their fungicidal activity. Expression of iNOS mRNA was increased after priming cells with each cytokine, and tended to be inhibited by Pb18. Despite up-regulation of iNOS mRNA expression by Pb265, an equivalent increase in NO production was not detected, as metabolite levels were similar in all cultures. The results indicated that high expression of human monocyte iNOS mRNA induced by P. brasiliensis is not correlated with NO concentrations produced.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Ana Paula Bordon-Graciani, Luciane Alarcão Dias-Melicio, Michele Janegitz Acorci-Valério, João Pessoa Jr., Angela Maria Victoriano de Campos Soares,