Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6135799 | Microbes and Infection | 2014 | 7 Pages |
Abstract
Rough Brucella mutants have been sought as vaccine candidates that do not interfere with the conventional serological diagnosis of brucellosis. In this study, a rough mutant of Brucella melitensis was generated by the disruption of the wzt gene, which encodes the O-polysaccharide (O-PS) export system ATP-binding protein. In vivo, the mutant 16MÎwzt was attenuated and conferred a level of protection against B. melitensis 16M challenge similar to that conferred by the vaccine strain B. melitensis M5 in mice. In pregnant sheep, the mutant 16MÎwzt did not induce abortion. In vitro, 16MÎwzt was more susceptible to polymyxin B and complement-mediated killing than B. melitensis 16M was. Most importantly, although 16MÎwzt had a rough phenotype, it was able to synthesize O-PS and did not induce detectable specific antibodies in sheep. These results suggested that 16MÎwzt deserved to further systematic evaluation as a vaccine for target animal hosts due to its promising features.
Keywords
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Immunology and Microbiology
Immunology
Authors
Zhen Wang, Jian Rui Niu, Xiao Lei Wang, Tong Lei Wu, Jie Cheng, Lin Lu, Qing Min Wu,