Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6137137 | Parasitology International | 2011 | 8 Pages |
Abstract
âºIn this manuscript Fasciola flukes were collected from different hosts (cattle, buffalo, and sheep) brought to different Abattoirs âºDNA was extracted and the target gene markers were amplified by PCR technique. âºObtained PCR fragments were directly sequenced or cloned into the appropriate vectors when necessary. âºNucleotide sequences were aligned and consensus sequences were subjected to blast homology search and phylogeny was inferred using maximum parsimony method as implemented in the MEGA 4 software based on mitochondrial gene markers NDI and COI. âºThis study provided the first molecular evidence of the presence of Fasciola hepatica/gigantica intermediate form in Egypt.
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Authors
Said Amer, Yasser Dar, Madoka Ichikawa, Yasuhiro Fukuda, Chika Tada, Tadashi Itagaki, Yutaka Nakai,