Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6138443 | Virology | 2016 | 11 Pages |
Abstract
Jumbo phages infecting Ralstonia solanacearum were isolated in Thailand (ÏRSL2) and Japan (ÏRSF1). They were similar regarding virion morphology, genomic arrangement, and host range. Phylogenetic and proteomic tree analyses demonstrate that the ÏRSL2 and ÏRSF1 belong to a group of evolutionary related phages, including Pseudomonas phages ÏKZ, 201Ï2-1 and all previously described ÏKZ-related phages. Despite conserved genomic co-linearity between the ÏRSL2 and ÏRSF1, they differ in protein separation patterns. A major difference was seen in the detection of virion-associated-RNA polymerase subunits. All β- and βâ²-subunits were detected in ÏRSF1, but one βâ²-subunit was undetected in ÏRSL2. Furthermore, ÏRSF1 infected host cells faster (latent period: 60 and 150 min for ÏRSF1 and ÏRSL2, respectively) and more efficiently than ÏRSL2. Therefore, the difference in virion-associated-RNA polymerase may affect infection efficiency. Finally, we show that ÏRSF1 is able to inhibit bacterial wilt progression in tomato plants.
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Authors
Anjana Bhunchoth, Romain Blanc-Mathieu, Tomoko Mihara, Yosuke Nishimura, Ahmed Askora, Namthip Phironrit, Chalida Leksomboon, Orawan Chatchawankanphanich, Takeru Kawasaki, Miyako Nakano, Makoto Fujie, Hiroyuki Ogata, Takashi Yamada,