Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6139009 | Virology | 2015 | 10 Pages |
Abstract
Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.
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Virology
Authors
Sue-Jane Lin, Shao-Wen Wu, Ya-Ching Chou, Jiun-Han Lin, Ya-Chi Huang, Mei-Ru Chen, Nianhan Ma, Ching-Hwa Tsai,