Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6139116 | Virology | 2015 | 10 Pages |
Abstract
Following the 2009 H1N1 pandemic, surveillance activities have been accelerated globally to monitor the emergence of novel reassortant viruses. However, the mechanism by which influenza A viruses of swine (IAV-S) acquire novel gene constellations through reassortment events in natural settings remains poorly understood. To explore the mechanism, we collected 785 nasal swabs from pigs in a farm in Thailand from 2011 to 2014. H3N2, H3N1, H1N1 and H1N2 IAVs-S were isolated from a single co-infected sample by plaque purification and showed a high degree of diversity of the genome. In particular, the H1N1 isolates, possessing a novel gene constellation previously unreported in Thailand, exhibited greater variation in internal genes than H3N2 IAVs-S. A pair of isolates, designated H3N2-B and H1N1-D, was determined to have been initially introduced to the farm. These results demonstrate that numerous IAVs-S with various gene constellations can be created in a single co-infected pig via reassortment.
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Authors
Haruka Abe, Junki Mine, Sujira Parchariyanon, Nobuhiro Takemae, Prakit Boonpornprasert, Namfon Ubonyaem, Phornnachat Patcharasinghawut, Bandit Nuansrichay, Taichiro Tanikawa, Ryota Tsunekuni, Takehiko Saito,