Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6139351 | Virology | 2015 | 9 Pages |
Abstract
Tobacco necrosis virus (TNV-D) has a plus-strand RNA genome that is neither 5â² capped nor 3â² poly-adenylated. Instead, it utilizes a 3â² cap-independent translational enhancer (3â²CITE) located in its 3â² untranslated region (UTR) for translation of its proteins. We have examined the protein expression strategies used by TNV-D and our results indicate that: (i) a base pairing interaction between conserved ACCA and UGGU motifs in the genomic 5â²UTR and 3â²CITE, respectively, is not required for efficient plant cell infection, (ii) similar potential 5â²UTR-3â²CITE interactions in the two viral subgenomic mRNAs are not needed for efficient translation of viral proteins in vitro, (iii) a small amount of capsid protein is translated from the viral genome by a largely 3â²CITE-independent mechanism, (iv) the larger of two possible forms of capsid protein is efficiently translated, and (v) p7b is translated from subgenomic mRNA1 by a leaky scanning mechanism.
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Immunology and Microbiology
Virology
Authors
Tamari Chkuaseli, Laura R. Newburn, David Bakhshinyan, K. Andrew White,