Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6139487 | Virology | 2015 | 13 Pages |
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins. Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells. Structural characterization of GP5 glycans would facilitate the mechanistic understanding of these processes. Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods. Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans. Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids. GlcNAc and LacNAc accessibility to ligands was confirmed by lectin co-precipitation. Our findings help to explain PRRSV infection of cells lacking sialoadhesin and provide a glycan database to facilitate molecular structural studies of PRRSV.
Keywords
PNGase FVirion structureLacNAcDSAWGASIAN-acetyllactosamineGP5LEAConAPRRSVNeuAcKdnGlcNAcNeuGcFucoseFucPeptide-N-glycosidase FConcanavalin ADatura stramonium agglutininN-GlycanN-acetylneuraminic acidN-glycolylneuraminic acidSialic acidMass spectrometryLectinN-acetylglucosaminePorcine reproductive and respiratory syndrome virusVeterinary virologyGalGalactoseWheat germ agglutinin
Related Topics
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Immunology and Microbiology
Virology
Authors
Juan Li, Shujuan Tao, Ron Orlando, Michael P. Murtaugh,