Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6139643 | Virology | 2015 | 10 Pages |
â¢Actin-myosin network is important for the influenza virus production.â¢HA forms aggregations at the plasma membrane in the presence of blebbistatin.â¢M1 is recruited to the budding site through the actin-myosin network.
Actin filaments are known to play a central role in cellular dynamics. After polymerization of actin, various actin-crosslinking proteins including non-muscle myosin II facilitate the formation of spatially organized actin filament networks. The actin-myosin network is highly expanded beneath plasma membrane. The genome of influenza virus (vRNA) replicates in the cell nucleus. Then, newly synthesized vRNAs are nuclear-exported to the cytoplasm as ribonucleoprotein complexes (vRNPs), followed by transport to the beneath plasma membrane where virus particles assemble. Here, we found that, by inhibiting actin-myosin network formation, the virus titer tends to be reduced and HA viral spike protein is aggregated on the plasma membrane. These results indicate that the actin-myosin network plays an important role in the virus formation.