Article ID Journal Published Year Pages File Type
6139643 Virology 2015 10 Pages PDF
Abstract

•Actin-myosin network is important for the influenza virus production.•HA forms aggregations at the plasma membrane in the presence of blebbistatin.•M1 is recruited to the budding site through the actin-myosin network.

Actin filaments are known to play a central role in cellular dynamics. After polymerization of actin, various actin-crosslinking proteins including non-muscle myosin II facilitate the formation of spatially organized actin filament networks. The actin-myosin network is highly expanded beneath plasma membrane. The genome of influenza virus (vRNA) replicates in the cell nucleus. Then, newly synthesized vRNAs are nuclear-exported to the cytoplasm as ribonucleoprotein complexes (vRNPs), followed by transport to the beneath plasma membrane where virus particles assemble. Here, we found that, by inhibiting actin-myosin network formation, the virus titer tends to be reduced and HA viral spike protein is aggregated on the plasma membrane. These results indicate that the actin-myosin network plays an important role in the virus formation.

Related Topics
Life Sciences Immunology and Microbiology Virology
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