Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6139844 | Virology | 2014 | 10 Pages |
Abstract
We have investigated whether reducing the overall size of adenovirus (Ad), through use of a vector containing a shortened fibre, leads to enhanced distribution and dissemination of the vector. Intravenous or intraperitoneal injection of Ad5SlacZ (12Â nm fibre versus the normal Ad5 37Â nm fibre) or Ad5SpKlacZ (shortened fibre with polylysine motif in the H-I loop of fibre knob domain) led to similar levels of lacZ expression compared to Ad5LlacZ (native Ad5 fibre) in the liver of treated animals, but did not enhance extravasation into the tibialis anterior muscle. Direct injection of the short-fibre vectors into the tibialis anterior muscle did not result in enhanced spread of the vector through muscle tissue, and led to only sporadic transgene expression in the spinal cord, suggesting that modifying the fibre length or redirecting viral infection to a more common cell surface receptor does not enhance motor neuron uptake or retrograde transport.
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Authors
Emily R. McFall, Lyndsay M. Murray, John A. Lunde, Bernard J. Jasmin, Rashmi Kothary, Robin J. Parks,