Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6140354 | Virology | 2014 | 12 Pages |
Abstract
Macrophages encounter flaviviruses early after injection by arthropod vectors. Using in vivo imaging of mice inoculated with firefly luciferase-expressing single-cycle flavivirus particles (FLUC-SCFV), we examined the initial dissemination of virus particles in the presence or absence of lymph node (LN)-resident macrophages. Higher luciferase activity, indicating higher SCFV gene expression, was detected in the footpad of macrophage-depleted mice after 24Â h post infection (hpi). Moreover, FLUC-SCFV particles disseminated to the spleen within 14Â hpi in macrophage-depleted, but not control mice. Although macrophages presented SCFV to naïve T cells in vitro, depletion of subcapsular sinus (SCS) macrophages did not alter the magnitude or effector function of the WNV-specific CD8+ T cell response. Together, these results indicate that SCS macrophages play a role in limiting the dissemination of SCFV early in infection but are not required for the generation of a polyfunctional WNV-specific CD8+ T cell response in the draining LN.
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Authors
Evandro R. Winkelmann, Douglas G. Widman, Jingya Xia, Alison J. Johnson, Nico van Rooijen, Peter W. Mason, Nigel Bourne, Gregg N. Milligan,