Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6140576 | Virology | 2014 | 7 Pages |
Abstract
Inflammasome activation is gaining recognition as an important mechanism for protection during viral infection. Here, we investigate whether Rift Valley fever virus, a negative-strand RNA virus, can induce inflammasome responses and IL-1β processing in immune cells. We have determined that RVFV induces NLRP3 inflammasome activation in murine dendritic cells, and that this process is dependent upon ASC and caspase-1. Furthermore, absence of the cellular RNA helicase adaptor protein MAVS/IPS-1 significantly reduces extracellular IL-1β during infection. Finally, direct imaging using confocal microscopy shows that the MAVS protein co-localizes with NLRP3 in the cytoplasm of RVFV infected cells.
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Authors
Megan E. Ermler, Zachary Traylor, Krupen Patel, Stefan A. Schattgen, Sivapriya K. Vanaja, Katherine A. Fitzgerald, Amy G. Hise,