Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6140713 | Virology | 2013 | 8 Pages |
Abstract
Recent investigations have shown the Egyptian fruit bat (Rousettus aegyptiacus) to be a natural reservoir for marburgviruses. To better understand the life cycle of these viruses in the natural host, a new reverse genetics system was developed for the reliable rescue of a Marburg virus (MARV) originally isolated directly from a R. aegyptiacus bat (371Bat). To develop this system, the exact terminal sequences were first determined by 5â² and 3â² RACE, followed by the cloning of viral proteins NP, VP35, VP30 and L into expression plasmids. Novel conditions were then developed to efficiently replicate virus mini-genomes followed by the construction of full-length genomic clones from which recombinant wild type and GFP-containing MARVs were rescued. Surprisingly, when these recombinant MARVs were propagated in primary human macrophages, a dramatic difference was found in their ability to grow and to elicit anti-viral cytokine responses.
Keywords
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Authors
César G. Albariño, Luke S. Uebelhoer, Joel P. Vincent, Marina L. Khristova, Ayan K. Chakrabarti, Anita McElroy, Stuart T. Nichol, Jonathan S. Towner,