Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6140792 | Virology | 2014 | 7 Pages |
â¢miR-24 and miR-205 are induced during keratinocyte differentiation.â¢This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7.â¢miR-205 is dependent upon pRb expression.â¢miR-24 targets p27 in cycling keratinocytes.
A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes.