| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6140843 | Virology | 2013 | 10 Pages |
â¢The Aedes albopictus-DENV-2 interactions were studied by 2D DIGE with MS analysis.â¢Partial mRNA sequences of differential proteins were obtained by sequencing.â¢Chaperone, cytoskeleton and energy metabolism enzyme were most changed post-infection.â¢mRNA distributions of differential proteins were the most abundant in salivary gland.
The interaction between dengue virus (DENV) and vector mosquitoes are still poorly understood at present. In this study, 2-D DIGE combined with MS was used to analyze the differential proteomes of Aedes albopictus salivary gland, midgut and C6/36 cells induced by DENV-2. Our results indicated that the virus infection regulated several functional classes of proteins. Among them, 26 were successfully analyzed by real-time RT-PCR. The mRNA levels of 15 were the highest in salivary gland, 2 in midgut and none in C6/36 cells, however, 18 were the least in fat body compared to other organs. Interestingly, the changes of differential proteins mRNA were the most obvious in fat body post-infection. Chaperone, cytoskeleton and energy metabolism enzyme were the most down- or up- regulated proteins after DENV-2 infection. The abundant expression of these proteins in salivary gland may relate to its high susceptibility.
