| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6141027 | Virology | 2013 | 8 Pages |
The Agrotis segetum granulovirus (AgseGV) F protein was previously identified as the first betabaculovirus F protein with functional homology to Autographa californica nucleopolyhedrovirus (AcMNPV) GP64. In the current study, F proteins from Xestia c-nigrum granulovirus (XecnGV), Cydia pomonella granulovirus (CpGV), Phthorimaea operculella granulovirus (PhopGV), Choristoneura occidentalis granulovirus (ChocGV) and Plutella xylostella GV (PlxyGV) were studied for their ability to rescue the infectivity of gp64-null AcMNPV. Our results showed that most studied betabaculovirus F proteins could replace the function of AcMNPV GP64, however, their efficiencies to rescue the infectivity of gp64-null AcMNPV were substantially different. PlxyF, although fusogenic, was the only protein that failed to substitute the function of AcMNPV GP64. Further studies using Sf90p1D cell line showed that PlxyF appeared to be properly incorporated into AcMNPV virions and underwent correct post-translational cleavage and N-linked glycosylation. However, the gp64-null AcMNPV containing PlxyF could not be propagated in either Sf9 or P. xylostella cells.
⺠Four out of five betabaculovirus F proteins studied can rescue gp64-null AcMNPV. ⺠The efficiency of F from different betabaculovirus to substitute for GP64 varies. ⺠PlxyGV F protein was unable to substitute GP64 in either Sf9 or Plxy cells. ⺠Cleavage and N-glycosylation of PlxyGV F protein appeared normal in Sf9OP1D cells.
