Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6141607 | Virology | 2010 | 6 Pages |
Abstract
Merkel cell polyomavirus (MCV) has been implicated as a causative agent in Merkel cell carcinoma. Robust polyclonal antibody responses against MCV have been documented in human subjects, but monoclonal antibodies (mAbs) specific for the VP1 capsid protein have not yet been characterized. We generated 12Â mAbs capable of binding recombinant MCV virus-like particles. The use of a short immunogenic priming schedule was important for production of the mAbs. Ten of the 12Â mAbs were highly effective for immunofluorescent staining of cells expressing capsid proteins. An overlapping set of 10Â mAbs were able to neutralize the infectivity of MCV-based reporter vectors, with 50% effective doses in the low picomolar range. Three mAbs interfered with the binding of MCV virus-like particles to cells. This panel of anti-capsid antibodies should provide a useful set of tools for the study of MCV.
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Authors
Diana V. Pastrana, Katherine A. Pumphrey, Nicolas Ãuburu, Rachel M. Schowalter, Christopher B. Buck,