Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6141796 | Virology | 2009 | 10 Pages |
Abstract
Human immunodeficiency virus type 1 (HIV-1) originated in chimpanzees; yet, several previous studies have shown that primary HIV-1 isolates replicate poorly in chimpanzee CD4+ T lymphocytes in vitro and in vivo. The reasons for this apparent restriction are not understood. Here, we describe a new activation protocol that led to a reproducible expansion and activation of chimpanzee CD4+ T lymphocytes in vitro. Using this protocol, we uncovered species-specific differences in the activation profiles of human and chimpanzee CD4+ T-cells, including HLA-DR and CD62L. Moreover, we found that improved activation facilitated the replication of both CXCR4 and CCR5-tropic HIV-1 in CD4+ T-cell cultures from over 30 different chimpanzees. Thus, the previously reported “replication block” of CCR5-tropic HIV-1 in chimpanzee lymphocytes appears to be due, at least in large part, to suboptimal T-cell activation.
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Authors
Julie M. Decker, Kenneth P. Zammit, Juliet L. Easlick, Mario L. Santiago, Denise Bonenberger, Beatrice H. Hahn, Olaf Kutsch, Frederic Bibollet-Ruche,