Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6142340 | Virus Research | 2014 | 8 Pages |
â¢A 35S promoter-driven infectious clone of PVX, pCaPVX100, was constructed.â¢Vector pCaPVX440 can express two foreign genes at the same time.â¢Plasmid pCaPVX760 can express foreign gene through gene substitution strategy.â¢Gene silencing vector pCaPVX440-LIC contains a ligation independent cloning site.
Multiple plant viruses, including potato virus X (PVX), have been modified as vectors for expressing heterologous genes or silencing endogenous genes in plants. PVX-based vectors facilitate the functional analysis of genes in plant. However, they can only express one protein in a time. In this paper we report the construction of new vectors based on a 35S promoter-driven PVX infectious clone, pCaPVX100. Vector pCaPVX440 contains two additional subgenomic promoters and can be utilized to express two foreign genes at the same time. Plasmid pCaPVX760 is a CP minus vector and can be used to express foreign proteins through the gene substitution strategy. In addition, plasmid pCaPVX100 was engineered into a gene silencing vector (pCaPVX440-LIC) by introducing a ligation independent cloning (LIC) site into the vector. These results indicate that the newly developed PVX vectors are competent for multiple research purposes.