Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6142485 | Virus Research | 2014 | 4 Pages |
Abstract
The encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect EMCV RNA. The RT-LAMP assay was highly sensitive and able to detect 2.2 Ã 10â5 ng of EMCV RNA, as no cross-reaction was observed with other viruses. The RT-LAMP assay was conducted in isothermal (62 °C) conditions within 50 min. The amplified products of EMCV could be detected as ladder-like bands using agarose gel electrophoresis. This is the first report to demonstrate the application of a one-step RT-LAMP assay for the detection of EMCV. The sensitive, specific and rapid RT-LAMP assay developed in this study can be applied widely in clinical diagnosis and field surveillance of EMCV.
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Authors
Wanzhe Yuan, Jianchang Wang, Mingtan Sun, Yingshuai Zheng, Limin Li, Xiuyuan Zhang, Jiguo Sun,