| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6142933 | Virus Research | 2013 | 15 Pages |
Abstract
⺠The HIV-1 nucleocapsid (NC) protein contains highly conserved aromatic amino acids. ⺠Mutating one or both aromatics in NC to Ala progressively reduces virus replication. ⺠NC DNA interaction kinetics are also reduced in Ala-mutant NC proteins. ⺠Helix destabilization by Ala-mutant NC proteins are similarly affected.
Keywords
repeat regionWLCssDNAquantitative reverse transcriptase PCRLTRnucleocapsidF16FJCGAGPAGEALAqRT-PCRFAMdsDNAcTAR5′UTRsingle stranded DNAdouble-stranded DNANucleic acid chaperonepolyacrylamide gel electrophoresisZinc fingerFluorescence anisotropyTarReverse transcriptaseLong terminal repeatSurface glycoproteinInfectivitywild typeERTpolymerase chain reactionPCRHIV-1Human immunodeficiency virus type 1ProteasecarboxyfluoresceinCapsid
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Authors
Hao Wu, Mithun Mitra, Micah J. McCauley, James A. Thomas, Ioulia Rouzina, Karin Musier-Forsyth, Mark C. Williams, Robert J. Gorelick,