| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6142940 | Virus Research | 2013 | 10 Pages |
Abstract
⺠Mechanism for removal of HIV-1 5â²-viral RNA fragments annealed to (â) strong-stop DNA. ⺠Removal most efficient with HIV-1 NC protein and functional RNase H. ⺠HIV-1 NC alone is sufficient, but must have helix destabilizing activity. ⺠Small RNA in duplex displaced by longer 3â² viral RNA in a strand exchange reaction. ⺠Common mechanism for RNA removal reactions in reverse transcription.
Keywords
(−) SSDNAtransactivation response elementHIV-1 nucleocapsid proteinZFSFAMPPT6-carboxyfluoresceinElectrophoretic mobility shift assayNucleic acid chaperoneFörster resonance energy transferFRETZinc fingersTarReverse transcriptaseRepeatSelf-primingPolypurine tractreverse transcriptionEMSA یا electrophoretic mobility shift assay wild-typeHIV-1Human immunodeficiency virus type 1Nucleocapsid protein
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Authors
Christopher B. Hergott, Mithun Mitra, Jianhui Guo, Tiyun Wu, Jennifer T. Miller, Yasumasa Iwatani, Robert J. Gorelick, Judith G. Levin,