Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6143006 | Virus Research | 2013 | 5 Pages |
Abstract
Serial passaging of yellow fever virus 17D in Vero cells was employed to derive seed material for a novel inactivated vaccine, XRX-001. Two independent passaging series identified a novel lysine to arginine mutation at amino acid 160 of the envelope protein, a surface-exposed residue in structural domain I. A third passage series resulted in an isoleucine to methionine mutation at residue 113 of the NS4B protein, a central membrane spanning region of the protein which has previously been associated with Vero cell adaptation of other mosquito-borne flaviviruses. These studies confirm that flavivirus adaptation to growth in Vero cells can be mediated by structural or non-structural protein mutations.
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Authors
David W.C. Beasley, Merribeth Morin, Ashley R. Lamb, Edward Hayman, Douglas M. Watts, Cynthia K. Lee, Dennis W. Trent, Thomas P. Monath,