Chimeric classical swine fever (CSF)-Japanese encephalitis (JE) viral replicon as a non-transmissible vaccine candidate against CSF and JE infections

► We constructed the E2-complemented CSF-JE virus replicon particle (VRP) that contains an antigenic gene of JEV in CSFV genome. ► We discovered that E2-complemented CSF-JE VRP has the potential as a live-attenuated non-transmissible vaccine candidate against CSF and JE but also serves as a potential DIVA (Differentiating Infected from Vaccinated Animals) vaccine for CSF in pigs. ► Our finding represented a promising strategy for the novel bivalent DIVA vaccine design.