Use of prophage free host for achieving homogenous population of bacteriophages: New findings

► A new methodology of making pure bacteriophage preparation using prophage free hosts is proposed. ► The role of phage endolysin in dictating host range of a phage is demonstrated. ► Release of prophages during phage multiplication in a prophage containing host is highlighted. ► We report efficiency of phage endolysin to play a role in effective phage propagation. ► Strength of endolysin for phage release in identification of a potent phage is speculated.