Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6143529 | Virus Research | 2011 | 9 Pages |
Abstract
We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain, FIPV 79-1146. We clarified that MAb used to prepare mar mutant viruses also lost its activity to enhance homologous mar mutant viruses, strongly suggesting that neutralizing and antibody-dependent enhancing epitopes are present in the same region in the strain FIPV 79-1146. We also discovered that amino acid mutation in the neutralizing epitope reduced viral replication in monocytes/macrophages. We also demonstrated that the mutation or deletion of two nucleotides in 7b gene abrogate the virulence of strain FIPV 79-1146.
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Authors
Tomomi Takano, Yoshika Tomiyama, Yasuichiroh Katoh, Michiyo Nakamura, Ryoichi Satoh, Tsutomu Hohdatsu,