Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6181773 | Fertility and Sterility | 2015 | 11 Pages |
Abstract
Bisphenol A (BPA) is a widely studied typical endocrine-disrupting chemical, and one of the major new issues is the safe replacement of this commonly used compound. Bisphenol S (BPS) and bisphenol F (BPF) are already or are planned to be used as BPA alternatives. With the use of a culture system that we developed (fetal testis assay [FeTA]), we previously showed that 10Â nmol/L BPA reduces basal testosterone secretion of human fetal testis explants and that the susceptibility to BPA is at least 100-fold lower in rat and mouse fetal testes. Here, we show that addition of LH in the FeTA system considerably enhances BPA minimum effective concentration in mouse and human but not in rat fetal testes. Then, using the FeTA system without LH (the experimental conditions in which mouse and human fetal testes are most sensitive to BPA), we found that, as for BPA, 10Â nmol/L BPS or BPF is sufficient to decrease basal testosterone secretion by human fetal testes with often nonmonotonic dose-response curves. In fetal mouse testes, the dose-response curves were mostly monotonic and the minimum effective concentrations were 1,000Â nmol/L for BPA and BPF and 100Â nmol/L for BPS. Finally, 10,000Â nmol/L BPA, BPS, or BPF reduced Insl3 expression in cultured mouse fetal testes. This is the first report describing BPS and BPF adverse effects on a physiologic function in humans and rodents.
Keywords
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Authors
Soria M.Sc., Tiphany M.Sc., Delphine M.Sc., Marie-Justine Ph.D., Thierry Ph.D., Stéphanie M.D., Alexandra M.D., Ph.D., Gabriel Ph.D., Virginie Ph.D., René Ph.D.,