Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6188622 | Reproductive BioMedicine Online | 2015 | 4 Pages |
Abstract
This study proposed a quantitative evaluation of oxidative status (OS) in bovine embryos. Sixteen-cell stage embryos, cultured under 5% O2, were treated with oxidative stress inducer menadione (0, 1, 2.5 and 5âµmol/l) for 24âh. Blastocyst rate (BLR) was recorded and expanded blastocysts were stained with CellROX®Green (CRG; OS evaluation) and evaluated under epifluorescence microscopy (ratio of pixel/blastomere). A significant effect of menadione was observed for BLR (P = 0.0039), number of blastomeres/embryo (P < 0.0001) and OS (P < 0.001). Strong negative correlations were found between BLR and the number of blastomeres with OS evaluation, demonstrating the efficacy of this analysis to evaluate OS levels of IVF bovine embryos.
Keywords
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Authors
Patricia Monken de Assis, Leticia Signori Castro, Adriano Felipe Perez Siqueira, Juliana de Carvalho Delgado, Thais Rose dos Santos Hamilton, Marcelo Demarchi Goissis, Camilla Mota Mendes, Marcilio Nichi, José Antonio Visintin,