Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6188849 | Reproductive BioMedicine Online | 2015 | 5 Pages |
Abstract
In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or 'negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool.
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Authors
Sara D.M. Valckx, Jessie De Bie, Ellen D. Michiels, Ilse G. Goovaerts, Usha Punjabi, Priscila Ramos-Ibeas, Alfonso Gutierrez-Adan, Peter E. Bols, Jo L. Leroy,