Association between ADAM12 polymorphism and knee osteoarthritis in Thai population

•The significant associations were shown from the C allele in male knee OA patients.•The CC genotype in male OA patients seemed to exhibit higher risk of knee OA than the GG genotype.•The rs3740199 in ADAM12 was not associated with the susceptibility of knee OA in the females.•The rs3740199 in ADAM12 was associated with knee OA susceptibility in Thai male patients.

BackgroundOsteoarthritis (OA), a common degenerative joint disorder in the elderly, is characterized by the destruction of articular cartilage, bony outgrowths at joint margins, and synovitis. The objective of this study was to evaluate whether there is an association between the ADAM12 (rs3740199) polymorphism and susceptibility to knee OA in a Thai population.MethodsGenomic deoxyribonucleic acid (DNA) was isolated from 200 Thai knee OA patients and 200 healthy controls. High resolution melting analysis was used to detect ADAM12 polymorphisms. The melt profile of all DNA samples was generated on the CFX96™ real-time polymerase chain reaction system and analyzed by Precision Melt Analysis™ software. The genotype distributions and allele frequencies of ADAM12 were compared between groups using the StatCalc program.ResultsThe significant associations were shown from the C allele (OR = 2.10, 95% CI = 1.16-3.79, P = 0.008) and the CC genotype (OR = 4.28, 95% CI = 1.21-15.72, P = 0.01) in male knee OA patients. No significant association was observed in female patients.ConclusionThe rs3740199 in ADAM12 was associated with knee OA susceptibility in Thai male patients, and individuals with the CC genotype carried the highest risk when compared with the GG and GC genotypes.Clinical relevanceThe rs3740199 polymorphism of the ADAM12 gene can potentially be used to determine genetically high-risk subgroup of knee osteoarthritis and to better understand the pathogenesis of knee osteoarthritis.