Research reportThe different effects of maternal separation on spatial learning and reversal learning in rats

•MS increased locomotor activity in the open field of all three ages of rats.•MS induced less anxiety-behaviors in the open field of adolescent rats.•MS slightly disrupted spatial learning of MWM in adolescent and young adult rats.•MS improved reversal learning of MWM in adolescent and young adult rats.

Early postnatal maternal separation (MS) can play an important role in the development of psychopathologies during ontogeny. In the present study, we investigated the effects of repeated MS (4 h per day from postnatal day (PND) 1 to 21) on locomotor activity and anxiety behavior in open field, spatial learning and reversal learning in Morris water maze of male and female juvenile (PND 21), adolescent (PND 35) and early adult (PND 56) Wistar rats. The results indicated that MS increased locomotor activity of rats across all ages and reduced anxiety behavior of adolescent rats in open field test. MS also increased swim distance in spatial learning and decreased escape latency in reversal learning in adolescent and early adult rats. Additionally, for socially reared rats, there was increased spontaneous locomotion with age, decreased reversal learning ability with age. The present study provides novel insights into the consequences of MS and demonstrates unique age-dependent changes at the behavioral levels.