Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6261940 | Brain Research Bulletin | 2012 | 12 Pages |
Methylphenidate (MPD) is a psychostimulant that enhances dopaminergic neurotransmission in the central nervous system by using mechanisms similar to cocaine and amphetamine. The mode of action of brain circuitry responsible for an animal's neuronal response to MPD is not fully understood. The nucleus accumbens (NAc) has been implicated in regulating the rewarding effects of psychostimulants. The present study used permanently implanted microelectrodes to investigate the acute and chronic effects of MPD on the firing rates of NAc neuronal units in freely behaving rats. On experimental day 1 (ED1), following a saline injection (control), a 30Â min baseline neuronal recording was obtained immediately followed by a 2.5Â mg/kg i.p. MPD injection and subsequent 60Â min neuronal recording. Daily 2.5Â mg/kg MPD injections were given on ED2 through ED6 followed by 3 washout days (ED7 to ED9). On ED10, neuronal recordings were resumed from the same animal after a saline and MPD (rechallenge) injection exactly as obtained on ED1. Sixty-seven NAc neuronal units exhibited similar wave shape, form and amplitude on ED1 and ED10 and their firing rates were used for analysis. MPD administration on ED1 elicited firing rate increases and decreases in 54% of NAc units when compared to their baselines. Six consecutive MPD administrations altered the neuronal baseline firing rates of 85% of NAc units. MPD rechallenge on ED10 elicited significant changes in 63% of NAc units. These alterations in firing rates are hypothesized to be through mechanisms that include D1 and D2-like DA receptor induced cellular adaptation and homeostatic adaptations/deregulation caused by acute and chronic MPD administration.
⺠Acute and chronic MPD administration modifies NAc neuronal firing rates. ⺠Acute administration of MPD elicits changes in firing rate to 54% of recorded NAc neuronal units. ⺠85% of NAc neuronal units exhibited an increased or decreased neuronal baseline after chronic MPD administration. ⺠Homeostatic changes and tolerance in NAc neuronal firing rates occur do to chronic administration of MPD.