| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6261980 | Brain Research Bulletin | 2011 | 7 Pages |
Neuronal nitric oxide synthase (nNOS) has been reported to be up-regulated in the hypothalamic supraoptic nucleus (SON) during dehydration which in turn could increase nitric oxide (NO) production and consequently affect arginine vasopressin (AVP) secretion. The anteroventral third ventricle (AV3V) region has strong afferent connections with the SON. Herein we describe our analysis of the effects of an AV3V lesion on AVP secretion, and c-fos and nNOS expression in the SON following dehydration. Male Wistar rats had their AV3V region electrolytically lesioned or were sham operated. After 21 days they were submitted to dehydration or left as controls (euhydrated). Two days later, one group was anaesthetized, perfused and the brains were processed for Fos protein and nNOS immunohistochemistry (IHC). Another group was decapitated, the blood collected for hematocrit, osmolality, serum sodium and AVP plasma level analysis. The brains were removed for measurement of neurohypophyseal AVP content, and the SON was punched out and processed for nNOS detection by western blotting. The AV3V lesion reduced AVP plasma levels and c-fos expression in the SON following dehydration (PÂ <Â 0.05). Western blotting revealed an up-regulation of nNOS in the SON of control animals following dehydration, whereas such up-regulation was not observed in AV3V-lesioned rats (PÂ <Â 0.05). We conclude that the AV3V region plays a role in regulating the expression of nNOS in the SON of rats submitted to dehydration, and thus may affect the local nitric oxide production and the secretion of vasopressin.
⺠Nitric oxide (NO) production may affect vasopressin secretion. ⺠nNOS is upregulated in the hypothalamic SON following dehydration. ⺠Anteroventral third ventricle region (AV3V) has strong connections with the SON. ⺠AV3V lesion prevents the nNOS up-regulation in the SON following dehydration. ⺠Prevention of nNOS up-regulation may affect vasopressin secretion.
