| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6262111 | Brain Research Bulletin | 2011 | 6 Pages |
Clasmatodendrosis is an irreversible astroglial degenerative change, which includes extensive swelling and vacuolization of cell bodies and disintegrated and beaded processes. Since alteration in F-actin level influences on the formation of vacuoles/vesicles during exocytosis/endocytosis in astrocytes, we investigated whether F-actin polymerization involves clasmatodendrosis in the rat hippocampus following status epilepticus (SE). In the present study, vacuoles in clasmatodendrotic astrocytes showed LAMP-1 and LC3-II (a marker for autophagy) immunoreactivity. These findings reveal that clasmatodendrosis may be lysosome-derived autophagic astroglial death. Jasplakinolide (an F-actin stabilizer) infusion significantly decreased the size and the number of medium/large-sized vacuoles in each clasmatodendritic astrocyte accompanied by enhancement of phalloidin signals, as compared to vehicle-infusion. In contrast, latrunculin A (an F-actin-depolymerizing agent) infusion increased the size and the number of medium/large-sized vacuoles, which were dissociated adjacent to cell membrane. Therefore, our findings suggest that F-actin stabilization may inhibit lysosome-derived autophagic astroglial death during clasmatodendrosis.
⺠Clasmatodendrosis is an irreversible astroglial degenerative changes. ⺠PLPP/CIN expression level was increased in clasmatodendritic astrocytes. ⺠Jasplakinolide infusion decreased vacuoles in clasmatodendritic astrocytes. ⺠Latrunculin A infusion increased vacuoles in clasmatodendritic astrocytes. ⺠F-actin polymerization involves clasmatodendrosis in the rat brain following SE.
