| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6264379 | Brain Research | 2012 | 14 Pages |
Corticosterone influences emotion and cognition via actions in a diversity of corticolimbic structures, including the amygdala. Since extracellular levels of corticosterone in brain have rarely been studied, we characterized a specific and sensitive enzymatic immunoassay for microdialysis quantification of corticosterone in the basolateral amygdaloid complex of freely-moving rats. Corticosterone levels showed marked diurnal variation with an evening (dark phase) peak and stable, low levels during the day (light phase). The “anxiogenic agents”, FG7142 (20Â mg/kg) and yohimbine (10Â mg/kg), and an environmental stressor, 15-min forced-swim, induced marked and sustained (1-3Â h) increases in dialysis levels of corticosterone in basolateral amygdaloid complex. They likewise increased dialysis levels of dopamine and noradrenaline, but not serotonin and GABA. As compared to basal corticosterone levels of ~Â 200-300Â pg/ml, the elevation provoked by forced-swim was ca. 20-fold and this increase was abolished by adrenalectomy. Interestingly, stress-induced rises of corticosterone levels in basolateral amygdaloid complex were abrogated by combined but not separate administration of the corticotrophin releasing factor1 (CRF1) receptor antagonist, CP154,526, and the vasopressin1b (V1b) receptor antagonist, SSR149,415. Underpinning their specificity, they did not block forced-swim-induced elevations in dopamine and noradrenaline. In conclusion, extracellular levels of corticosterone in the basolateral amygdaloid complex display marked diurnal variation. Further, they are markedly elevated by acute stressors, the effects of which are mediated (in contrast to concomitant elevations in levels of monoamines) by co-joint recruitment of CRF1 and V1b receptors.
⺠Corticosterone levels were quantified in basolateral amygdaloid complex dialysates. ⺠These levels of corticosterone showed diurnal variation with a marked nocturnal peak. ⺠Environmental and pharmacological stressors elevated these levels of corticosterone. ⺠Stress induces rise in corticosterone blunted by blockade of CRF1 and V1b receptors.
