| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6269197 | Journal of Neuroscience Methods | 2012 | 9 Pages |
Abstract
⺠We developed a method for measuring the endogenous concentration of a protein in small cellular compartments in brain tissue. ⺠The method involves overexpression of the protein of interest which is fused with a fluorescent protein such as GFP. ⺠Overexpression of a soluble fluorescent protein with shifted emission (RFP) is also needed for demarcating the cell volume. ⺠It also requires immunostaining and high-resolution fluorescence confocal or two-photon imaging. ⺠The method was applied for measuring the endogenous concentration of CaMKII in cell soma, dendrites, and dendritic spines.
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Authors
Nikolai Otmakhov, John Lisman,