| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6269360 | Journal of Neuroscience Methods | 2013 | 14 Pages |
Abstract
⺠We describe a method for studying the axonal transport of endogenous proteins fused to HaloTag® in rat sympathetic neurons in compartmented cultures. ⺠Applying HaloTag ligand to the distal axons allows the tracking of labeled proteins during retrograde transport. ⺠We demonstrate that the transport of HaloTag protein alone does not interfere with tracking the transport of HaloTag fusion proteins. ⺠We demonstrate the retrograde transport of HaloTag protein fused to the p75 neurotrophin receptor. ⺠We also demonstrate the anterograde transport of HaloTag protein fused to tubulin.
Keywords
SDSHaloTagtetramethylrhodaminep75NTRNGFPAGETMRPBSBDNFDMSOpolyacrylamide gel electrophoresisRetrograde transportAnterograde transportDimethylsulfoxidesodium dodecyl sulfateRetrograde signalingbrain derived neurotrophic factornerve growth factorPhosphate-buffered salineNeurotrophinSympathetic neuronsp75 neurotrophin receptor
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Authors
Sue-Ann Mok, Karen Lund, Paul LaPointe, Robert B. Campenot,