Multimodal vessel mapping for precise large area alignment of functional optical imaging data to neuroanatomical preparations in marmosets

► We present a new technique for improving accuracy of neuroanato-functional alignment. ► By multimodal vessel mapping, optical imaging maps are aligned to large cortical regions. ► Spatial inaccuracies e.g. tissue distortions can be considerably reduced. ► We demonstrate the influence of cortical curvature onto accuracy of alignment. ► The dislocation of functional signals to histological landmarks is quantified.