A method to solubilise protein aggregates for immunoassay quantification which overcomes the neurofilament “hook” effect

▶ Plasma neurofilament (Nf) levels may be a biomarker of neurodegenerative disease. ▶ Nf aggregates cause a 'hook effect' that prevents accurate quantification by ELISA. ▶ A new method to dissolve Nf aggregates and abolish the hook effect was developed. ▶ The method is sensitive, accurate and reproducible. ▶ Detects pathological increases in Nf levels in plasma of mSOD1 mice that model ALS.