Single-sweep voltage-sensitive dye imaging of interacting identified neurons

The simultaneous recording of many individual neurons is fundamental to understanding the integral functionality of neural systems. Imaging with voltage-sensitive dyes (VSDs) is a key approach to achieve this goal and a promising technique to supplement electrophysiological recordings. However, the lack of connectivity maps between imaged neurons and the requirement of averaging over repeated trials impede functional interpretations. Here we demonstrate fast, high resolution and single-sweep VSD imaging of identified and synaptically interacting neurons. We show for the first time the optical recording of individual action potentials and mutual inhibitory synaptic input of two key players in the well-characterized pyloric central pattern generator in the crab stomatogastric ganglion (STG). We also demonstrate the presence of individual synaptic potentials from other identified circuit neurons. We argue that imaging of neural networks with identifiable neurons with well-known connectivity, like in the STG, is crucial for the understanding of emergence of network functionality.

Research highlights▶ Voltage-sensitive dye imaging of the crab stomatogastric ganglion is demonstrated. ▶ Single-sweep imaging of interacting identified neurons in a central pattern generator. ▶ High spatial resolution recording of sub-threshold neural events is demonstrated. ▶ The importance of imaging networks with known neurons and connectivity is discussed.