Seizure logging: A new approach to synchronized cable-free EEG and video recordings of seizure activity in mice

We describe a new cable-free, non-telemetric method for synchronized electrophysiological and video recordings of seizure activity in freely moving mice. The electrophysiological recordings were made by a head-mounted 4-channel data-logging device, allowing the mouse to move freely in its cage, and even to be moved from cage to cage under ongoing recording. Seizures were studied in Synapsin I/II double knock-out (SynDKO) mice, a genetically engineered mouse line that shows seizures upon daily handling procedures such as tail lifting during cage changes, much in resemblance to the more studied El mouse. The ability to elicit seizures through daily handling in SynDKO mice undergoing electrophysiological recording is a significant improvement in comparison to the traditional cable-based set-up. Furthermore, with its four channels and a sample rate of up to 500 Hz, the data-logging device opens for more varied electrophysiological studies than other available cable-free systems.

Research highlights▶ A cable-free head-mounted data logger recorded mouse handling evoked seizure EEGs. ▶ Logged seizures were provoked in the same manner as non-logged control seizures. ▶ Logged-EEG/Video synchronization allowed detailed seizure behavior analysis.