Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6271035 | Neuroscience | 2016 | 11 Pages |
Abstract
Dopamine and glutamate play critical roles in the reinforcing effects of cocaine. We demonstrated that a single intraperitoneal administration of cocaine induces a significant decrease in [3H]-d-aspartate uptake in the pre-frontal cortex (PFC). This decrease is associated with elevated dopamine levels, and requires dopamine D1-receptor signaling (D1R) and adenylyl cyclase activation. The effect was observed within 10Â min of cocaine administration and lasted for up to 30Â min. This rapid response is related to D1R-mediated cAMP-mediated activation of PKA and phosphorylation of the excitatory amino acid transporters EAAT1, EAAT2 and EAAT3. We also demonstrated that cocaine exposure increases extracellular d-aspartate, l-glutamate and d-serine in the PFC. Our data suggest that cocaine activates dopamine D1 receptor signaling and PKA pathway to regulate EAATs function and extracellular EAA level in the PFC.
Keywords
dl-threo-β-benzyloxyaspartateDHKPFCD1RHEPESTTBSEGTAPKCEAADATEAATs4-(2-hydroxyethyl)-1-piperazineethanesulfonic aciddl-TBOAexcitatory amino acidEDTAethylene glycol tetraacetic acidEthylenediaminetetraacetic acidDopamine transporteranalysis of varianceANOVAminimal essential mediumTransporterDopaminedihydrokainic acidprefrontal cortexpre-frontal cortexMEMProtein kinase Chigh-performance liquid chromatographyHPLCCocaineglutamate
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Matheus Figueiredo Sathler, Bernardo Stutz, Robertta Silva Martins, MaurÃcio dos Santos Pereira, Ney Roner Pecinalli, Luis E. Santos, Rosilane Taveira-da-Silva, Jennifer Lowe, Isis Grigorio de Freitas, Ricardo Augusto de Melo Reis, Alex C. Manhães,