Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6271660 | Neuroscience | 2015 | 9 Pages |
Abstract
The present study aimed to examine the effects of chronic social defeat stress on the dopamine receptors and proteins involved in post-endocytic trafficking pathways. Adult mice were divided into susceptible and unsusceptible groups after 10Â days of social defeat stress. Western blot analysis was used to measure the protein expression levels of dopamine D2 receptors (D2Rs), a short (D2S) and a long form (D2L) and, D2R monomers and dimers, dopamine D1 receptors (D1Rs), neuronal calcium sensor-1 (NCS-1) and G protein-coupled receptor-associated sorting protein-1 (GASP-1), and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA expression levels of D2S, D2L, D2R monomers and dimers, and D1Rs in different brain areas. We observed increased expression of D2S, D2L and D2Rs dimers in the prefrontal cortex (PFC) of susceptible and/or unsusceptible mice compared with controls. The only significant findings with regard to mRNA expression levels were lower expression of D2S mRNA in the amygdala (AMYG) of susceptible and unsusceptible mice compared with controls. The present study demonstrated that chronic social defeat stress induced increased expression of D2S, D2L, and D2R dimers in the PFC of susceptible and/or unsusceptible mice.
Keywords
PBSPFCNCS-1EGTAAFBHRPRT-PCRAMYGGPCRsDLPFCG protein-coupled receptor kinaseG protein-coupled receptorsAmygdalaEDTAethylene glycol tetraacetic acidStriatumethylenediaminetetra acetic acidNeuronal calcium sensor-1Social defeatprefrontal cortexdorsolateral prefrontal cortexPhosphate-buffered salineHIPHippocampusreverse transcription-polymerase chain reactionHorseradish peroxidaseD1 receptorsD2 receptors
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Authors
T.R. Bagalkot, H.-M. Jin, V.V. Prabhu, S.S. Muna, Y. Cui, B.K. Yadav, H.-J. Chae, Y.-C. Chung,